Sandwiches and Surface Markers: How to Analyze Flow Cytometry Data

Sandwiches and Surface Markers: How to Analyze Flow Cytometry Data

Every cell in an organism has unique functions which require specific proteins. For example, cells in eyes need proteins that can detect light, and immune cells use a variety of different proteins to detect and kill invading pathogens. To categorize each type of cell from a big group (say, from a blood sample, which contains many different cells), we can classify them based on their proteins. Now imagine you want to look at many different proteins on a lot of individual cells, and you want to do it fast. How can a scientist analyze almost twenty proteins on each cell, from millions of single cells, in less than an hour? This isn’t the imaginary dream of a tired graduate student, or a magical machine available only to the richest labs­—this is flow cytometry. 

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